L-11 Three-dimensional reconstruction in electron microscopy

Life Sciences

Three-dimensional (3D) structural studies of macromolecules and cells have redefined our understanding of the molecular architecture of cells. Electron tomography has become a powerful tool for studying molecular and supramolecular structures in unperturbed functional environments, i.e. intact cells. By examining samples embedded in vitreous ice, structures are preserved in a close-to-life state. In this session, the focus will be on methodology including sample preparation, tomographic data acquisition, three-dimensional reconstructions of molecules and cells as well as image analysis and visualization.

Chairpersons:

Invited Speakers:
- Julio Ortiz, Max Planck Institute for Biochemistry, Martinsried, Germany
- Dorit Hanein, Sanford, Burnham Medical Research Institute, USA